Development and validation of a rapid selective high-throughput LC-MS/MS method for the determination of triclabendazole sulfoxide concentrations in sheep plasma and its use in bioequivalence studies

Abstract Triclabendazole is one of the main drugs used to treat liver fluke in livestock. A rapid LC-MS/MS method was developed and validated determine ovine plasma levels triclabendazole sulfoxide. Gemini NX-C18 column achieve analytical separation, with gradient elution a mobile phase composed 0.1% formic acid acetonitril water at flow rate 0.6 mL/min. MRM positive ESI ionization for detection sulfoxide ( m/z 360.10 from 376.97). Fenbendazole as internal standard. Plasma protein precipitation acetonitrile sample processing. The regards selectivity, linearity (r > 0.9939), within run between precision (CV < 8.9%) accuracy (bias over concentration range 1–100 µg/mL plasma. simple, selective can be applied bioequivalence bioavailability studies.